Part:BBa_K1838006
MLC Glucose Sensitive Yellow Chromoprotein Construct
This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-sensitive chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of an average strength promoter when placed upstream of the base promoter sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 30
Illegal NheI site found at 53 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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